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pcdna™6.2/c‐emgfp‐gw/topo™ only (empty vector [ev], control)  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pcdna™6.2/c‐emgfp‐gw/topo™ only (empty vector [ev], control)
    Pcdna™6.2/C‐Emgfp‐Gw/Topo™ Only (Empty Vector [Ev], Control), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/pmc06590123-74-18-10
    Average 90 stars, based on 1 article reviews
    pcdna™6.2/c‐emgfp‐gw/topo™ only (empty vector [ev], control) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Hsp70 regulation on Nox4/p22phox and cytoskeletal integrity as an effect of losartan in vascular smooth muscle cells
    Article Snippet: For the knockdown of Hsp72 expression, transient transfections were done with 2 μg/ml shHsp72-pSIREN-RetroQ vector and pSIREN-RetroQ empty vector (mock transfection control, empty vector (EV)) for 72 h using Lipofectamine TM 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s recommendations.

    Article Title: Losartan through Hsp70 Avoids Angiotensin II Induced Mesenchymal Epithelial Transition in Proximal Tubule Cells from Spontaneously Hypertensive Rats.
    Article Snippet: Briefly, for knockdown of Hsp72 expression, transient transfections were done with 2 μg/ml shHsp72-pSIREN-RetroQ vector and/or pSIREN-RetroQ empty vector (ev) (Mock-transfection control) for 48 h using LipofectamineTM2000 (Invitrogen, Carlsbad, CA) according to manufacturer’s recommendations.

    Transfection:

    Article Title: Targeting the LOX/hypoxia axis reverses many of the features that make pancreatic cancer deadly: inhibition of LOX abrogates metastasis and enhances drug efficacy
    Article Snippet: .. KP fl C cells were transfected with 1 μg of pCEF empty vector control plasmid DNA (EV) or pCEF full-length LOX-containing plasmid (LOX H1) using Lipofectamine (Invitrogen) for 48 h. Cells were then transferred into 1 mg/ml G418 (Invitrogen) medium for single-cell clone selection. ..

    Article Title: Targeting the LOX/hypoxia axis reverses many of the features that make pancreatic cancer deadly: inhibition of LOX abrogates metastasis and enhances drug efficacy.
    Article Snippet: .. KPflC cells were transfected with 1 lg of pCEF empty vector control plasmid DNA (EV) or pCEF full-length LOX-containing plasmid (LOX H1) using Lipofectamine (Invitrogen) for 48 h. Cells were then transferred into 1 mg/ml G418 (Invitrogen) medium for single-cell clone selection. ..

    Control:

    Article Title: Targeting the LOX/hypoxia axis reverses many of the features that make pancreatic cancer deadly: inhibition of LOX abrogates metastasis and enhances drug efficacy
    Article Snippet: .. KP fl C cells were transfected with 1 μg of pCEF empty vector control plasmid DNA (EV) or pCEF full-length LOX-containing plasmid (LOX H1) using Lipofectamine (Invitrogen) for 48 h. Cells were then transferred into 1 mg/ml G418 (Invitrogen) medium for single-cell clone selection. ..

    Article Title: Targeting the LOX/hypoxia axis reverses many of the features that make pancreatic cancer deadly: inhibition of LOX abrogates metastasis and enhances drug efficacy.
    Article Snippet: .. KPflC cells were transfected with 1 lg of pCEF empty vector control plasmid DNA (EV) or pCEF full-length LOX-containing plasmid (LOX H1) using Lipofectamine (Invitrogen) for 48 h. Cells were then transferred into 1 mg/ml G418 (Invitrogen) medium for single-cell clone selection. ..

    Plasmid Preparation:

    Article Title: Targeting the LOX/hypoxia axis reverses many of the features that make pancreatic cancer deadly: inhibition of LOX abrogates metastasis and enhances drug efficacy
    Article Snippet: .. KP fl C cells were transfected with 1 μg of pCEF empty vector control plasmid DNA (EV) or pCEF full-length LOX-containing plasmid (LOX H1) using Lipofectamine (Invitrogen) for 48 h. Cells were then transferred into 1 mg/ml G418 (Invitrogen) medium for single-cell clone selection. ..

    Article Title: Targeting the LOX/hypoxia axis reverses many of the features that make pancreatic cancer deadly: inhibition of LOX abrogates metastasis and enhances drug efficacy.
    Article Snippet: .. KPflC cells were transfected with 1 lg of pCEF empty vector control plasmid DNA (EV) or pCEF full-length LOX-containing plasmid (LOX H1) using Lipofectamine (Invitrogen) for 48 h. Cells were then transferred into 1 mg/ml G418 (Invitrogen) medium for single-cell clone selection. ..

    Single Cell:

    Article Title: Targeting the LOX/hypoxia axis reverses many of the features that make pancreatic cancer deadly: inhibition of LOX abrogates metastasis and enhances drug efficacy
    Article Snippet: .. KP fl C cells were transfected with 1 μg of pCEF empty vector control plasmid DNA (EV) or pCEF full-length LOX-containing plasmid (LOX H1) using Lipofectamine (Invitrogen) for 48 h. Cells were then transferred into 1 mg/ml G418 (Invitrogen) medium for single-cell clone selection. ..

    Article Title: Targeting the LOX/hypoxia axis reverses many of the features that make pancreatic cancer deadly: inhibition of LOX abrogates metastasis and enhances drug efficacy.
    Article Snippet: .. KPflC cells were transfected with 1 lg of pCEF empty vector control plasmid DNA (EV) or pCEF full-length LOX-containing plasmid (LOX H1) using Lipofectamine (Invitrogen) for 48 h. Cells were then transferred into 1 mg/ml G418 (Invitrogen) medium for single-cell clone selection. ..

    Selection:

    Article Title: Targeting the LOX/hypoxia axis reverses many of the features that make pancreatic cancer deadly: inhibition of LOX abrogates metastasis and enhances drug efficacy
    Article Snippet: .. KP fl C cells were transfected with 1 μg of pCEF empty vector control plasmid DNA (EV) or pCEF full-length LOX-containing plasmid (LOX H1) using Lipofectamine (Invitrogen) for 48 h. Cells were then transferred into 1 mg/ml G418 (Invitrogen) medium for single-cell clone selection. ..

    Article Title: Targeting the LOX/hypoxia axis reverses many of the features that make pancreatic cancer deadly: inhibition of LOX abrogates metastasis and enhances drug efficacy.
    Article Snippet: .. KPflC cells were transfected with 1 lg of pCEF empty vector control plasmid DNA (EV) or pCEF full-length LOX-containing plasmid (LOX H1) using Lipofectamine (Invitrogen) for 48 h. Cells were then transferred into 1 mg/ml G418 (Invitrogen) medium for single-cell clone selection. ..



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    Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its <t>empty</t> <t>vector</t> (EV) <t>control</t> (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)
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    Image Search Results


    Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its empty vector (EV) control (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)

    Journal: Arthritis research & therapy

    Article Title: Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis.

    doi: 10.1186/s13075-017-1388-8

    Figure Lengend Snippet: Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its empty vector (EV) control (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)

    Article Snippet: Adenoviruses for transient expression of LOXL2 (AdCMV-RFP-CMV-hLOXL2-His; referred to as Ad-RFPLOXL2), and its empty vector (EV) control (Ad-RFP-EV) were custom-synthesized (ADV-214438) by Vector Biolabs.

    Techniques: Transduction, Expressing, Plasmid Preparation, Control, Activity Assay